Journal: Molecular Biology Reports

Article Title: Porphyromonas gingivalis -derived lipopolysaccharide confers chemotherapy resistance and migratory ability on oral cancer cells by activating toll-like receptor 4 signaling pathway

doi: 10.1007/s11033-026-11713-1

Figure Lengend Snippet: NF-κB activation in P. g -LPS-treated OM-1 cells. ( A ) Western blot showing the expression of NF-κB/p65 and phosphorylation of NF-κB/p65 in P. g -LPS-treated OM-1 cells and P.g -LPS + TAK-242-treated cells. The ratio of the band density of phospho-NF-κB/p65 protein to that of NF-κB/p65 (** P < 0.01, *** P < 0.001, one-way ANOVA with post-hoc Tukey’s HSD test). ( B ) Immunofluorescence staining of NF-κB/p65 (green) and nuclear counterstaining with 4′,6-diamidino-2-phenylindole (DAPI) (blue) in control OM-1 cells, P. g -LPS-treated cells, and P. g -LPS + TAK-242-treated cells. ( C ) Cytotoxicity assay in SC75741-treated OM-1 cells. ( D ) Cytotoxicity assay in P. g -LPS + SC75741-treated OM-1 cells in the presence of 5-FU (*** P < 0.01, one-way ANOVA with post-hoc Tukey’s HSD test). ( E ) Relative expression ratio of BAX / BCL2 mRNA in P. g -LPS + SC75741-treated OM-1 cells in the presence of 5-FU (*** P < 0.01, one-way ANOVA with post-hoc Tukey’s HSD test). ( F ) Cytotoxicity assay in P. g -LPS + SC75741-treated HOC621 cells in the presence of 5-FU (* P < 0.05, unpaired t-test)

Article Snippet: NF-κB p65 mouse monoclonal antibody (Proteintech) and Alexa Fluor 488 goat anti-mouse antibody were used for NF-κB p65 staining.

Techniques: Activation Assay, Western Blot, Expressing, Phospho-proteomics, Immunofluorescence, Staining, Control, Cytotoxicity Assay